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KMID : 0613820070170040568
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Journal of Life Science
2007 Volume.17 No. 4 p.568 ~ p.574
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Partial Characterization and Induction of Ferulic Acid Esterase and Xylanase from Pseudomonas sp. LG2
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Kim Yong-Gyun
Lee Sang-Mong
Park Hyean-Cheal
Kim Keun-Ki
Son Hong-Joo
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Abstract
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Lignin degrading bacterium Pseudomonas sp. LG2 was able to degrade lignin substrate to a lot of APPL compound. APPL compound was detected in culture supernatants from Pseudomonas sp. LG2 grown with BSG(brewer’s spent grain). FAE(ferulic acid esterase) and xylanase are induced from Pseudomonas sp. LG2 in the presence of carbon sources such as oat spelt xylan, HBSG Ⅰ, Ⅱ(hydrolyzed brewer’s spent grainⅠ, Ⅱ) and AFBSG(autoclaved fraction from brewer’s spent grain). However, xylanase and FAE are not induced by growth of Pseudomonas sp. LG2 on xylose and arabinose. Pseudomonas sp. LG2 is grown on medium containing oat spelt xylan, HBSG Ⅰ, Ⅱ and AFBSG and the induction of FAE and xylanase activities of extracellular proteins determined during 14 days. Maximum level of xylanase activity(5.3 U/㎎) found at 6 days in culture contained oat spelt xylan as carbon source, whereas maximum level of FAE activity(15.4 mU/㎎) was found at 8 days in culture contained AFBSG as carbon source. Most ferulic acid was released in culture supernatants when Pseudomonas sp. LG2 grown on oat spelt xylan, HBSG Ⅰ, Ⅱ and AFBSG. FAE of extracellular enzymes was also specific activity on methyl ferulic acid, methyl caffeic acid and methyl p-coumaric acid respectively, but not methyl sinapinic acid, methyl vanillic acid and methyl gallic acid.
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KEYWORD
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Pseudomonas sp. LG2, ferulic acid esterase, xylanase
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